Biomimetic Technology Research Group (BTRG)
Horizon Europe keywords:
· Health technologies, new tools and solutions
· Pharmaceutical technologies
Faculty of Chemical Technology and Biotechnology Department of Chemical and Environmental Process Engineering
Introduction of the Research Group
The BTRG research group is primarily concerned with the study of the in vitro ADME and physicochemical properties of active pharmaceutical agents, drug formulations, and development of related biomimetic models.
Main methods belonging to our activity:
- kinetic and thermodynamic solubility and dissolution tests
- investigation of the solubility conditions of active substances, especially through the action of certain excipients (eg cyclodextrins)
- pKa measurement by UV-pH titration method
- logP measurement by equilibrium distribution and HPLC methods
- in vitro tissue-specific (blood-brain barrier (BBB), gastrointestinal, cornea, nasal, liver and heart) lipid membrane permeability models: PAMPA and side-bi-side systems
- HSA binding study by rapid equilibrium dialysis and specific HPLC system
- chemical stability measurement in physiological buffer systems (PBS, SGF, SIF, Fassif, Fessif)
- metabolic stability and intrinsic clearance study in the liver microsome system
- CyP inhibition assay
- production of an oxidative metabolite in a metalloporphyrin-based flow chemical system
- investigation of biological oxidation transformations in electrochemical systems
- development and testing of in silico ADME and PD models (supporting software: ACD / Percepta, Marvin Sketch)
Achievements
- Fine-tuning and development of tissue-specific PAMPA models: BBB, buccal, corneal, nasal- Development of an in silico QSPR model for corneal permeability
- Application of PAMPA systems in dereplication of natural products from plants
- Investigation of the interaction between active substances and macromolecules based on changes in proton dissociation processes (pKa): drug-serum albumin and drug-cyclodextrin systems
- Synthesis and investigation of oxidative drug metabolites using continuous-flow biomimetic systems (liver-on-a-chip)
Publications
1. Gyarmati, Benjámin ; Dargó, Gergő ; Szilágyi, Barnabás Áron ; Vincze, Anna ; Facskó, Réka ; Budai-Szűcs, Mária ; Kiss, Eszter L. ; Szente, Lajos ; Szilágyi, András✉; Balogh, György T.✉ Synthesis, complex formation and corneal permeation of cyclodextrin-modified, thiolated poly(aspartic acid) as self-gelling formulation of dexamethasone. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS 174 pp. 1-9. , 9 p. (2022)2. Vincze, Anna ; Dargó, Gergő ; Rácz, Anita ✉ ; Balogh, György T. ✉:A corneal-PAMPA-based in silico model for predicting corneal permeability. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 203 p. 114218 Paper: 114218, 10 p. (2021)
3. Dargó, G; Bajusz, D; Simon, K; Müller, J; Balogh, GyT✉: Human serum albumin binding in a vial: a novel UV-pH titration method to assist drug design. JOURNAL OF MEDICINAL CHEMISTRY 63(4) pp. 1763-1774. (2020)
4. Balogh-Weiser, D; Decsi, B; Krammer, R; Dargó, G; Ender, F; Mizsei, J; Berkecz, R; Gyarmati, B; Szilágyi, A; Tőtős, R; Paizs, Cs; Poppe, L; Balogh, GyT✉: Magnetic Nanoparticles with Dual Surface Functions—Efficient Carriers for Metalloporphyrin-Catalyzed Drug Metabolite Synthesis in Batch and Continuous-Flow Reactors. NANOMATERIALS 10 Papers 2329. (2020)
5. Fődi, T; Ignácz, G; Decsi, B; Béni, Z; Túrós, Gy I; Kupai, J; Balogh-Weiser, D; Greiner I; Huszthy, P; Balogh GyT.✉: Biomimetic synthesis of drug metabolites in batch and continuous‐flow reactors. CHEMISTRY-A EUROPEAN JOURNAL 24 :37 pp. 9385-9392. (2018)
Patents
1. Nagy, ZZs;, Balogh, GyT; Katona, G; Csorba, A; Kiss, H; Zupkó, I; Csóka, I: Postoperative application of the ASP eye drop formula. Date: 2021 2. Balogh, GyT; Illés, J; Boros, A; Forrai, E; Székely, Á: Pharmaceutical composition comprising a zinc-hyaluronate complex for the treatment of multiple sclerosis. WO 2005049047, Date:: 2005Journals
Major international journals: Pharmaceutics, EurJPharmSci, JMedChem, JPBAOral and poster presentation at domestic and international conferences (examples from the last 5 years):
2022 PSCR (Osaka), 2020 Ocean4Biotech COST Action CA18238 (Malta), 2018 7th IACP and PCF-J Meeting (Osaka), 2017 RICT (Toulouse)
Infrastructure
- PAMPA and side-bi-side permeability systems - 96-well plate based solubility measurement system - HPLC, LC-TQMS - preparative HPLC system - UV-vis plate reader - electrochemical systemsProjects
The head of the BTRG research group is the lead researcher:2021– CELSA 2021/019, BME-KU-Leuven, Development of In silico Absorption Models by Machine Learning Method
2020– Cooperative Doctoral Program Application, NKFIH, 1010984, Chemical Library Planning in Pharmaceutical Research
2020– TKP-2020. NKA-05, Intensification of Chemical Technologies, Development of High Permeability Biometric Systems Subgroup
The head of the BTRG research group is the co-researcher:
2016–2021 OTKA K119770
2020– OTKA K134704
Industry relations
- Waters Research Center- Mcule Ltd
- EGIS Pharmaceutical Plc
- Servier Kutatóközpont
- Cyclolab Llc
- Solvo Biotechnológia - Charles River Co.
- ThalesNano Inc
- Spinsplit Llc
- MolTeam Llc
Conferences
2022 PSCR (Osaka), GyTBalogh, session chair2020 Ocean4Biotech COST Action CA18238 (Malta) GyTBalogh, invited lecturer
2018 7th IACP and PCF-J Meeting (Osaka) GDargó, poster presentation
2017 RICT (Toulouse) GyTBalogh, poster presentation